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[Difco]BPA(Baird Parker Agar) 500g (010175)

BAIRD PARKER AGAR BASE

276840 / 500g

Directions for Preparation from

Dehydrated Product

1. Suspend 63 g of the powder in 950 mL of purified water. Mix thoroughly.

2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.

3. Autoclave at 121°C for 15 minutes.

4. Cool to 45-50°C and aseptically add 50 mL of EY Tellurite Enrichment. Mix thoroughly but gently.

5. Test samples of the finished product for performance using stable, typical control cultures.

Procedure

Food samples are macerated in suitable broth medium, diluted as desired and the dilutions

spread-inoculated onto the agar surfaces, which should be dry when inoculated.

Incubate plates aerobically for 24 hours at 35 ± 2°C. Consult references for detailed instructions.

Expected Results

Typical colonies of S. aureus are black, shiny, convex and surrounded by clear zones

(egg yolk reaction) of approximately 2-5 mm. Coagulase-negative staphylococci generally

do not grow well; if some growth occurs, the typical clear zones are absent.

The majority of other organisms are inhibited but some may grow sparsely,

producing white to brown colonies with no clearing of the egg yolk.